Finally the dependency on sample history is investigated. Thesis, massachusetts institute of technology, cambridge, ma, usa, june 2001. Equation 2 shows that the dep force on an object depends upon its volume. He taught me to write about the cinema, corrected and published my. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Dep can also be used for sample preparation 34, molecular. The dielectrophoresis 2016 meeting will take place at the massachusetts institute of technology in july 2016. This force does not require the particle to be charged.
Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying dep to unmet. Development of microsystems for the controlled formation of cell. Angela inmaculada lopezlorente, miguel valcarcel, in comprehensive analytical chemistry, 2014. A good basic understanding of the various acelectrokinetic effects is also provided by gimsa 2012. Pdf on the dielectrophoretic particle retention in porous media. Polarization and manipulation at liquidliquid interfaces by mitchell desmond a thesis submitted to johns hopkins university in conformity with the requirements for the degree of master of. In this thesis, i present a theory for charged nanocolloid dielectrophoresis taking into account the surface charge and debye double layer effects.
Our expertise is in the development of lowcost, robust technology for dielectrophoresis appications, and in. The quantities cm and r are the effective capacitance of the membrane and the radius of the cell respectively, while. Bakewell and others published dielectrophoresis of. Thereafter, every pleasant thing that happened in my life lowed to him. Design and simulation of a novel dielectrophoresis based. All testing sites were exposed to the same fluid sample.
On the relationship of dielectrophoresis and electrowetting. Figure 1 illustrates the behaviors of particles in the nonuniform electric field. Proofof principle results presented in this thesis demon. This meeting will build on the exciting progress made during the first ever dielectrophoresis meeting held in london during the summer of 2014. Electrical forces such as electrophoresis ep and dielectrophoresis dep are the subtle solution to manipulate particles in loc devices due to their favorable scaling for the reduced size of the system 100. Pdf dielectrophoresis of colloids and polyelectrolytes. The motion occurs due to the polarization of natural particles, which then experience a force called the dielectrophoretic force 62.
A critical step in the successful dna analysis of most sexual assault cases is the. Insulatorbased dielectrophoretic manipulation of dna in a microfluidic device by lin gan a dissertation presented in partial fulfillment of the requirements for the degree doctor of philosophy approved july 2015 by the graduate supervisory committee. The nonuniform electric field is formed by applying ac voltage between a pin electrode and a plate electrode. Pdf on the dielectrophoretic particle retention in. An ids system usually consist of a rf frequency voltage source up to 24 v, syringe pumps, microscope, and computer. Pianar electrode dielectrophoresis systems lee fason hartley a thesis submitted to the faculty of graduate studies in partial, fulfillment of the reqwdements for the degree of master of science department of electrical and computer engineering calgary, alberta september, 1997 o lee fason hartley 1997. Biological cell separation using dielectrophoresis in a microfluidic device r. Dielectrophoresis summary of models for dna for dep short dna 150 bp stiff rod 1 coiled sphere 2 maxwellwagnerokonski mwo theory. Yoyo, resulting in final concentrations of 200 ngml dna 300 nm bp, 200 nm yoyo, 0. As an example of the growing importance of dielectrophoresis, two special issues focused on dielectrophoresis were published by the journal electrophoresis in september 2011 2, 3. Eindhoven university of technology master dielectrophoresis in. A dissertation presented in partial fulfillment of the requirements for the degree doctor of philosophy.
Insulatorbased dielectrophoresis for bacterial characterization and trapping diana nakidde thesis submitted to the faculty of the virginia polytechnic institute and state university in partial fulfillment of the requirements for the degree of master of science in electrical engineering masoud agah, chair kwangjin koh chenming zhang. Development of a procedure for dielectrophoretic dep. An abstract of the thesis of oregon state university. The removal of chargeneutral particles by the principle. The application of dielectrophoresis to biological materials is also useful in analyzing phenomena of natural electric oscillation pohl, 1983, cell rotation zimmermann and arnold, 1983, cellular spin resonance mischel and lamprecht, 1980, cellular orientation teixeirapinto et al. Taking an interdisciplinary approach, it covers aspects of the theory and practice of dielectrophoresis concerned with. This phd thesis is the result of a collaboration between two laboratories. Dielectrophoresis for bioparticle manipulation mdpi. Numerical modeling of dielectrophoretic effect for. This phd dissertation describes experimental and theoretical. A critical step in the successful dna analysis of most sexual assault cases is the effective separation of male sperm and female epithelial cells, which are typically collected on a vaginal swab at the hospital soon after the event. Due to their high surface volume ratio, surface interactions can be a problem with micro and nanoparticles, and thus a chapter on the basics of surface forces has also been included. The application of dielectrophoresis in cell cycle studies by nafiseh naeemikhondabi a thesis submitted to the university of surrey in candidature for the degree of. Electrophoresis 1 trapping of dna by dielectrophoresis 2.
Pdf the theory of dielectrophoresis and its applications. The application of dielectrophoresis in cell cycle studies. Dielectrophoresis often abbreviated dep was first observed early in the 20th century, but described fully and named herbert pohl in 1950. In eq 17, e d is the uniform electric field in the dielectric layer. Pdf in the 1950s, pohl 1951, 1958 described dielectrophoresis as the movement of neutral particles under the influence of nonuniform electric. Equation 3 has the same form as 1 and the effective moment is defined by comparing these two expressions. Insulatorbased dielectrophoresis idep provides an efficient and matrixfree approach for manipulation of microand. The two curves correspond to force plots calculated with. This special topic section is on dielectrophoresis, a growing area of widespread interest and relevance to the microfluidics and nanofluidics community. Isodielectrophoresis ids can be used to characterize and separate cells by their electrical characteristics. These affect the particles dipole, and allow us to determine the dielectric properties such as resistance and capacitance. Simulation of dielectrophoretic assembly of carbon. We do this by observing particle motion at several ac frequencies, rather than at dc, and inferring the properties from. This thesis focus in dielectrophoretic approach due.
In paper 2, coding and calculations were done by yl, with the feedback from. Taking an interdisciplinary approach, it covers aspects of the theory and practice of dielectrophoresis concerned with characterizing and manipulating cells. Dielectrophoresis and electrowetting langmuir, vol. Simulation of dielectrophoretic assembly of carbon nanotubes using 3d. Yl did the coding and major part of calculation and the modeling part. Numerical modeling of dielectrophoresis yuan lin diva portal. Insulatorbased dielectrophoretic manipulation of dna in a.
Modeling of dielectrophoresis in micro and nano systems. The theory of dielectrophoresis and its applications on medical and materials research article pdf available december 2016 with 786 reads how we measure reads. Integration of vaporsolid grown zno nanowires through dielectrophoresis. However, the strength of the force depends strongly on the. The dielectrophoresis group at the university of surrey was established in 1999. Insulatorbased dielectrophoretic manipulation of dna in a microfluidic device lin gan 07172015. Dielectrophoretic precipitation of airborne particles. The aes electrophoresis society is pleased to launch the premier online resource for dielectrophoresis and the multitude of specialty subtopics. Bahrami2 1 department of electrical and computer engineering, university of tabriz, iran 2 ece department, university of tabriz, iran abstract. Alexandra ros, chair daniel buttry yan liu arizona state university august 2015. Payen university of california, berkeley bio and thermal engineering laboratory eecs 245 abstract basic ic fabrication techniques are employed in this paper with the purpose of designing a micro. Dielectrophoresis is the unidirectional motion of neutral, polarizable material which results from the presence of a nonuniform d. Foreword by franfois trufjaut andre bazin wrote about film better than anybody else in europe. This document contains a student thesis bachelors or masters, as authored by a student at eindhoven university of technology.
The motion occurs due to the polarization of natural particles, which then experience a force called the dielectrophoretic. The principles behind electrorotation, dielectrophoresis, and other electrokinetic effects are described in section 2. Dielectrophoresis is defined as the motion of a neutral particle caused by polarization effects in a nonuniform electric field1, 2. Pdf in the 1950s, pohl 1951, 1958 described dielectrophoresis as the. The dep force depends on the target particles volume and on the.
The uid dynamics involved and the dielectric properties of water inside singlewalled carbon nanotube are studied as well. Dielectrophoresis assembling cancer cells in a 3d microfluidic model. Dielectrophoresis dep is a phenomenon in which a force is exerted on a dielectric particle when it is subjected to a nonuniform electric field. Materials and methods fresh samples of normal blood of volume nearly 2 ml of different groups were collected from lions club blood bank. Dielectrophoresis is the electrokinetic motion of particles that occurs when a polarizable particle is placed in nonuniform electric fields, and the particle motion is influenced by the ambient electric field and by the properties of the dielectric particles or solutions lee et al. Dielectrophoresis and its application to biomedical. Modeling of dielectrophoresis in micro and nano systems yuan lin 2008 kth mechanics se100 44 stockholm, sweden abstract this thesis presents models and simulations of dielectrophoretic separation of micro and nano particles. When the dielectric constant of particle is larger than that of.
A study based on comsol multiphysics modeling a thesis presented to the graduate school of clemson university in partial fulfillment of the requirements for the degree master of science bioengineering by vandana devi pandian babu august 20 accepted by. Design and simulation of a novel dielectrophoresis based device to separate breast normal and cancerous cells m. Therefore, for a given ionic strength, the volume of the double layer will increase with the square of the particle radius. Polarization and manipulation at liquidliquid interfaces by mitchell desmond a thesis submitted to johns hopkins university in conformity with the requirements for the degree of master of science engineering baltimore, maryland august, 2014. Materials and methods fresh samples of normal blood of volume nearly 2 ml of different groups were collected from lions club blood bank, hyderabad. It is an electrostatic phenomenon, distantly related to electrophoresis, and describes the motion of suspended particles resulting from polarisation forces produced by an inhomogeneous electric field. Theory, methodology and biological applications describes the significant advances in the theory, technology and biomedical applications of dielectrophoresis since herbert pohls seminal monograph of 1978. Dielectrophoresis in lossy dielectric media nature. Dielectrophoresis is similar but uses a shaped ac field to determine the dielectric properties. It has produced nearly 80 papers in the field of dielectophoresis and related phenomena, three books and six patents and have been cited over 5500 times. Onchip separation of spermatozoa using dielectrophoresis. All particles exhibit dielectrophoretic activity in the presence of. Dielectrophoresis an overview sciencedirect topics. The instantaneous part can be characterized by the dielectric.
This thesis presents an active microarray that exploits electrokinetic electrophoresis and dielectrophoresis forces for its hybridization method using 3d carbon electrodes that will enable the large volume manipulation for pathogen detection. The dep force exerted on the cell is given by 2 3 2. Jun 29, 2010 this special topic section is on dielectrophoresis, a growing area of widespread interest and relevance to the microfluidics and nanofluidics community. Equation 2 has been applied by pohl 1978 to spherical yeast cells. The exact mechanism of the origin of the stern layer, through the surface conductance effect of a nanocolloid to form a collapsed diffuse layer that renders a nanocolloid conductive at sub. This masters thesis is brought to you for free and open access by. Dielectrophoresis dep is defined as the motion of neutral particles due to the application of an external nonuniform electric field. If a particle is suspended in an electric field that. In the differential extraction procedure currently used by most forensic dna analysts, the swab. Rapid communication dielectrophoretic separation of. The thickness d of the double layer increases with decreasing ionic concentration of the suspending medium but is independent of the particle size. Dielectrophoresis and rotation of cells springerlink. From that day in 1948 when he got me my first film job, working alongside him, i became his adopted son. Electrophoresis 2002, 23, 26582666 were made immediately prior to use by mixing these two solutions with deionized water in the proportion 47.